Research Associate, Protein Biochemistry

Job Locations US-MA-Cambridge
Job Post Information* : Posted Date 5 months ago(2/14/2020 2:43 PM)
ID
2019-1256
# of Openings
1
Category
Research & Development
Location : Address
10 Wilson Road

Overview

Deerfield Management is an investment management firm committed to improving healthcare through investment, information and philanthropy. We have just launched a new company called Civetta Therapeutics located in Cambridge, MA that was founded by William Sellers (Broad Institute and Dana-Farber Cancer Institute) and Eric Fischer (Dana Farber Cancer Institute and Harvard Medical School). We are looking to engage a high-quality team of researchers to advance new medicines through small molecule intervention targeting propeller domains with the goal of developing important therapeutics for cancer and other indications. If you are a talented life sciences professional that can bring significant energy, passion and talent to the team, we welcome the opportunity to consider you for a position in this exciting, new company.


About the Founders:

William Sellers is a core institute member of the Broad Institute of MIT and Harvard, a faculty member at Harvard Medical School, and faculty member and senior advisor to the president for experimental therapeutics at the Dana-Farber Cancer Institute.
Eric Fischer is an Assistant Professor in the Department of Biological Chemistry and Molecular Pharmacology at Harvard Medical School and an Independent Investigator, Dana-Farber Cancer Institute.

Responsibilities

In this role, part of the Lead Discovery team at Civetta, you will work under the supervision of a Senior Scientist to implement a pipeline for expression and purification of human proteins in both bacterial and insect cell systems. Your skills in cell culture and protein purification will be critical to experiments designed to provide protein reagents for downstream work in high-throughput screening and structural biology at Civetta. This is an excellent opportunity to learn and grow in the unique environment of a start-up biotech in Cambridge.

  

Key Responsibilities:

  • Perform small-scale and pilot-scale expression trials using robotic liquid handling systems to identify conditions for soluble expression of human proteins in heterologous systems
  • Scale-up to liter volumes of bacteria in high-density bio-fermentation systems, and of insect cells in shaking incubators
  • Learn and implement standardized purification workflows for multiple-column purifications of fusion-tagged proteins

Qualifications

Required Skills and Qualifications:

  • MS or BS degree in biochemistry, molecular/cell biology, or equivalent with 1-2 years of laboratory experience
  • Solid experience with standard molecular biology techniques including PCR, primer design, and subcloning into expression vectors preferred.
  • Exposure to protein biochemistry techniques in small scale, for example analysis of soluble expression using Western blotting and/or small-scale purification of protein utilizing affinity resins, is preferred.
  • Exposure to Opentrons or other robotic liquid handling systems, or willingness to learn these tools, is a strong plus.
  • Familiarity with standard gravity column purification procedures (i.e. nickel or GST affinity) and protein SDS-PAGE gel analysis desired. Exposure to protein chromatography systems (Bio-Rad NGC or GE AKTA systems) highly desirable.
  • Good attention to detail, and excellent time management, to enable use of electronic data notebooks and database software in a timely manner to track/annotate plasmid and protein stocks
  • Pride in performing high-quality, reproducible results, and willingness to communicate weekly and monthly scientific progress to team members on an informal basis
  • Eager to work on tight deadlines and troubleshoot/optimize protocols within those deadlines to deliver quality reagents to internal stakeholders

Civetta Therapeutics provides equal employment opportunities (EEO) to all employees and applicants for employment without regard to religion, race, creed, color, sex, sexual orientation, alienage or citizenship status, national origin, age, marital status, pregnancy, disability, veteran or military status, predisposing genetic characteristics or any other characteristic protected by applicable federal, state or local law.

 

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